Make sure the setup for Tb Cryptate is used. Bench top solutions for cell based assays. 10 μL of GLP1 receptor cells were dispensed into wells of. For more information. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Smallest and most affordable multimode plate reader in the industry. Suggested plate map: 1: 2 3: A: 14 μL Std 0: 3 μL IP1 d2 Reagent 3 μL IP1 Tb Cryptate AntibodyCisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. FOR HTRF CERTIFIED READER For. 1 – 1. White 384-well low volume plate Make sure to use the set-up for Eu3+ Cryptate. For more information about HTRF. The multidetection microplate readers CLARIOstar, PHERAstar FS, FLUOstar Omega, and POLARstar Omega are all HTRF-certified by Cisbio, meaning each of the. The Dual-Luciferase ® Reporter Assay or DLR is widely used to study gene transcription and regulation. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Incubate overnight at RT. Fluorescence intensity – Fluorescence intensity is one of the most popular detection modes, and there are innumerable fluorophores available for applications such as DNA or protein quantitation, reporter-geneCertified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. Even if not listed, some readers may be compatible with HTRF technology. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option. Plate 25 μL of cells in. NON-SPECIFIC BINDING TOTAL BINDINGCover the plate with a plate sealer. 45mm 5000µs Delay time Reading time Operation mode 100 µs 400 µs by plate 100 µs 400 µs by plate • This reader only allows high performance HTRF measurement when assays are run in WHITE plates. 3. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: ONE-PLATE ASSAY PROTOCOL: overnight * * Depending on cell lines used, volume of lysis should be optimized. Remove the plate sealer and read on an HTRF® compatible reader . HTRF is a FRET-based technology that emerged from the Jean-Marie Lehn ’s Nobel prize-winning work on rare earth complexes. For more information about set-up and compatible HTRF® readers,The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. No washing steps are required. CLARIOstar is equipped with a specific optical device which enables the measurement of. Read other about this measurement approach here. The two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates into a 384-well low volume detection plate before the addition of Total Cereblon HTRF detection reagents. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:320/40 HTRF Eu cryptate 665/7uv Wide Filter 0. Both Microplate Optimization and Read Height Adjustment were. Catalog No. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. * For HTRF microplate recommendations, please visit . HTRF Signal + 16 µL Standard or Sample 4 µL of pre-mixed TNFα Antibodies ADD READ ANALYSE Incubate 2 hours* at room temperature *Following incubation, the signal remains stable over a period of 24 hours. 5 µL Prostaglandin E2 . Incubate overnight at RT. Make sure the setup for Eu3+ Cryptate is used. Lysates are then transferred to the assay plate for the detection of total-EGFR by HTRF. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. The Firefly reaction is initiated, followed by its quenching and the subsequent initiation of the Renilla reaction. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Whatever the plate type, assay volume should match well volume as closely as possible so that focalization of the reader excitation beam gives maximum energy delivery (Fig. Seal the plate and incubate 3 hours at RT . PerkinElmer. Thanks to HTRF signal stability, the assay plate can be used to check several instruments, or different reader. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. **For more information about HTRF® compatible readers and for set-up recommendations, please visit our website at:ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Specialized filters allow for IR fluorescence. FOR HTRF CERTIFIED READER. After an overnight incubation, the plate is washed 3 times. Set up your reader for Eu3+ Cryptate and read the fluorescence emission at two different wavelengths (665nm and 620nm) on a compatible HTRF® reader**. CLARIOstar is equipped with a specific optical device which enables the measurement of. This protocol, HTS designed, allows miniaturization while. For more information. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit our. The CLARIOstar® Plus is our most flexible multi-mode plate reader. FOR HTRF CERTIFIED READER. best fluorescence polarization plate reader on the market. • HTRF®-Certified Reader **. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:The PHERAstar FS is an advanced HTS plate reader designed for all HTRF assays and features the following: All microplate formats up to 3456-well. FOR HTRF CERTIFIED. FOR HTRF CERTIFIED READER. Make sure the setup for Tb Cryptate is used. Plates can be loaded manually or plates can be loaded using 20 or 50-plate free access magazines which fit in a stacker. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Based on this customer experience and feedback, the Infinite 200 PRO microplate plate reader has been continuously engineered for excellence. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Compared to ELISA technology, MSD assays: Require far less sample. Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. The EnVision XCite Plate Reader provides exceptional sensitivity and speed with minimal downtime - backed by our global network of application scientists and engineers. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Its unique optical design and instant polarizer switching provide the smallest mP standard deviation in any assay. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Eu cryptate emission level). It features a modular design that allows users to add or change detection cartridges according to their needs. Plate 25 μL of cells in. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. Read & analyse. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. FOR HTRF CERTIFIED. For more information about HTRF ®The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER. The VICTOR Nivo is a HTRF certified microplate reader. An HTRF assay measures the fluorescence energy transfer between molecules in close proximity. Plate 25 μL of cells in. FOR HTRF CERTIFIED READER. • Select Excitation Filter 320x40 HTRF Eu cryptate* • Select Excitation Optic Empty • Select Emission Filter 495xm10uv • Select Emission Optic 2 – FPThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Plus . The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. AlphaLISA SureFire Ultra results are expressed as “Alpha counts”, and HTRF results were expressed as DeltaF, calculated according to CisBio instructions. The mean and standard deviation. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. FlexStation® 3 Multi-Mode Microplate Reader. FOR HTRF CERTIFIED. FOR HTRF CERTIFIED. For more information about microplate recommendations, please visit our website • Store the kit at -16°C. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance forThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Remove the plate seal and read on an HTRF compatible reader. Plate 25 μL of cells in. Remove the plate seal and read on an HTRF compatible reader. Each well generally requires 50µL of lysis buffer 1X. The tried, tested, and proven EnVision plate reader has an array of exciting features that can enhance your applications. For more information about HTRF. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. PerkinElmer’s 96w low volume, 96w and 384w microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. The DLR assay is a two step reaction that uses two luciferase enzymes, Firefly and Renilla (Figure 1). Partnerships for delivering turn-key solutions. Place the imaging filter cube on top of the LED cube you installed, and use the hex wrench to screw it to the LED cube. ** The Detection Buffer is used to prepare working solutions of acceptor and donor reagents. HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. HTRF® Europium cryptate donor / Red acceptor readout Setup recommendations for Tristar 5FOR HTRF CERTIFIED READER. For more information about HTRF. Lysates are then transferred to the assay plate for the detection of total-RET by HTRF. FOR HTRF CERTIFIED READER. For more. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. FOR HTRF CERTIFIED READER. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. Preconfigured HTRF. Make sure the plate reader is turnedon and then open the MikroWin software on the computer. Your lab might even have one already. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: + 10 µL lysis buffer ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. FOR HTRF CERTIFIED READER. For a list of HTRF-compatible readers and set-up recommendations, please visit . com HTRF Microplate Readers. Figure 1. Our microplates and accessories are manufactured under strict process controls guaranteeing consistent product performance for. • HTRF®-Certified Reader. (In the following text, the term YTHDC1 is used frequently as an abbreviation for the m 6 A-recognition domain of YTHDC1). For multiplexed luminescent and fluorescent assays, using a white plate will support maximum light output signal for the luminescent portion of the assay, but result in higher crosstalk and background for the fluorescent portion of the assay. These plates can be read on all HTRF certified readers, thus making their implementation in. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. Incubate 2h at RT Transfer 16 µL supernatant. Make sure the setup for Tb Cryptate is used. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. Remove the plate sealer and read on an HTRF® compatible reader . * For HTRF microplate recommendations, please visit Table 1 Instrument settings used for HTRF with terbium donor and red acceptor. It enables you to gain insights you couldn’t achieve with detection measurements alone. 96-well culture plateFOR HTRF CERTIFIED READER. LANCE ® Ultra DELFIA ® Certified HTRF Readers and Setups Already more than 50 certified or compatible instruments, and counting! HTRF is compatible with a large. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. 3 and higher include optimized reader settings, as well as data analysis, for faster results. • HTRF®-Certified Reader. CLARIOstar is equipped with a specific optical device which enables the measurement of both 620 nm cryptate and 665 nm acceptor emissions. Handle complex biological matrices easily. An. It is used as a positive control for Gs applications ( biological models validation,Detection of Total CDK12 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. • HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. The controls are equivalent to the upper and lower limit of. Incubate overnight at RT. Because of its homogeneous and fast nature, the assay is a preferred method to determine the protein concentration of samples. Time-resolved fluorescence was measured switch the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). Unlike fluorescence intensity which focuses on the quantification of emission intensity at a specific wavelength and neglects its polarization, fluorescence polarization specifically analyses as output the emission intensity of. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. FOR HTRF CERTIFIED READER. Incubate 4h at RT. DLReady™ certified luminometer plate readers. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Your lab might even have one already. Not for use in diagnostic procedures. FOR HTRF CERTIFIED READER. Plate 25 μL of cells in. Read & analyse. If not, our scientific team will help you choose the instrument that best suits your needs and budget. Read & analyse. Lysates are then transferred to the assay plate for the detection of Total-eNOS by HTRF. 5 hours before a further 3 washing steps. Our assay development platform relies on PHERAstar readers for absorbance, fluorescence, luminescence, and homogeneous time resolved. • HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. These plates can be read on all HTRF certified readers, thus making their implementation in. Make sure the setup for Tb Cryptate is used. Signals are detected at two different wavelengths (typically 620 nm and 665 nm), which are used to. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. Make sure the setup for Eu Cryptate is used. + 4 µL HTRF pre-mixed antibodies. Plate cells Stimulate cells 3. Plate 25 μL of cells in. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. CulturPlate-96: White Opaque 96-well Microplate, Sterile and Tissue Culture Treated. Its. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Manufacturer. The detection antibody is incubated for 1. For a list of HTRF-compatible readers and set -up recommendations, please visit. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. Incubate overnight at RT. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Taking these considerations into account, Cisbio’s 96w low volume and PKI’s 96w & 384w microplates have been carefully designed to provide the best signal detection environment possible to all HTRF assays. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes. 96-well culture plateFOR HTRF CERTIFIED READER. This protocol gives the cells viability and confluence to be monitored. This is thanks to a dedicated Alpha Technology laser which outperforms any xenon-based light source by exciting donors at 680 nm. You can verify your reader’s capacity to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. Incubate overnight at RT. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your. 5 μL of Binding buffer 2X. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Lysates are then transferred to the assay plate for the detection of total-EGFR by HTRF. It can also be further streamlined to a one-. FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. For more information about HTRF ® compatible readers and for set-up recommendations, please. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. The plate was covered both incubated for one hour at room temperature. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. The optimized well design and high quality plastics provides low background and crosstalk. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. White 384-well low volume plate Make sure to use the set-up for Eu 3+ Cryptate. For HTRF certified reader For more information about HTRF® compatible readers and for set- up recommendations, please visit our website. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. FOR HTRF CERTIFIED READER. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. With its ease of use software and remote access from almost any device, it is ideal for multiuser environments. FOR HTRF CERTIFIED READER. Please note that this kit use a Terbium donor crypate, make sure to set up your reader for Terbium cryptate. Utilizing HTRF (Homogeneous Time-resolved Fluorescence) technology, the assay enables simple and rapid characterization of compound and antibody blockers in a high throughput format. Simply place the module into one of the five module bays and read the assay. FOR HTRF CERTIFIED READER For more. Push the screw down and turn it clockwise to lock the plate. February 23, 2010, WINOOSKI VT, USA - The Synergy TM H4 Hybrid Microplate Reader from BioTek Instruments is now HTRF® certified. The optional HTRF-certified filter-based module may be. For more information about HTRF ®The LUMIstar® Omega is BMG LABTECH's powerful dedicated luminescence microplate reader, providing highly sensitive flash and glow assay readings. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: + 10 µL lysis buffer ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. pre+ 5 µL HTRFcompounds Activation Buffer. HTRF certified . At Cisbio we provide microplates that help you discover better. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. These plates can be read on all HTRF certified readers, thus making their implementation in. . 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Remove the plate seal and read on an HTRF compatible reader. It can also be further streamlined to a one-. Read & analyse. It measures at any wavelength, with top and bottom reading for most standard technologies. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. Conclusion. For more information about HTRF ®Excitation range. Learn about the principles, applications, and benefits of HTRF in this Agilent application note. 96-well culture plateHTRF certified . They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Detection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. Plate 25 μL of cells in. For more information about HTRF. It continues to deliver optimal results. 5. As shown in Figure 1, the interaction between Tag1-SOS1 and Tag2-KRAS-WT is detected by using anti-Tag1-Terbium (HTRF donor) and anti-Tag2-XL665 (HTRF acceptor). COMPONENTS ; STORAGE . Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Discover our high performance reader. Lysates are then transferred to the assay plate for the detection of total-p53 by HTRF. 6% for 1,408 wells and a Z’ value of 0. Incubate overnight at RT. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Lyse cells Read on an HTRF compatible reader . e. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. For more information about HTRF ®Highly compatible plates for HTRF. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. MATERIALS PROVIDED: KIT . The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within theassay linear range 6 Add 4 μL of premixed antibody solutions (vol/vol) prepared in the detection buffer. 1) . First the plate is coated with a capture antibody. NC1286225. Plate 25 μL of cells in. 8 µL cells. -CERTIFIED READER IS NEEDED. Cell seeding densities of 2,000,000 cells/well are generally sufficient for most cell lines, but. Sensitive, accurate and reliable detection is critical across cell, protein and genomic analysis applications in drug discovery and development. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Highly compatible plates for HTRF. High-end TR-FRET/HTRF® performance: certified ®to measure HTRF assays in both black or white plates, the reader guarantees no compromises in any HTRF assay. No washing steps are required. Read & analyse. View Brochure. Rating: 5. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed. spectramax paradigm system, htrf reagents, spectramax i3 and paradigm readers, HTRF assay, htrf certifiedLysates are then transferred ®to the assay plate for the detection of Total CHK2 by HTRF reagents. The Negative control is used to check the non-specific signal. Read & analyse. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Read & analyse. Incubate 4h at RT. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. It accepts all types of microtitration plate. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. These plates can be read on all HTRF certified readers, thus making their implementation in. To perform the assay, use white plate only. 5 µL. and TR-FRET, for example to detect PerkinElmer’s DELFIA, HTRF and LANCE assay technologies Time resolved fluorescence certified for use with HTRF. The LVis Plate is transparent in the UV-range of light and is compatible with all BMG LABTECH microplate readers. SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4 SpectraMax® M5 Multi-Mode Microplate Reader MLDVM5 SpectraMax® M5e Multi-Mode Microplate Reader (HTRF certified) MLDVM5E To prevent evaporation during assay incubation steps, we recommend the use of TopSeal-A PLUS, a clear adhesive seal for microplate. It can also be further streamlined to TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Small volume detection plate 96-half well plate. For more information about HTRF. Other technologies such as imaging. HTRF Signal + 2 hours @ RT [Cortisol] ng/ml 10 µL Standard or Sample 5 µL Cortisol acceptor reagent 5 µL Cortisol donor antibody ADD READ & ANALYSESafire 2™ readers must be appropriately be configured for HTRF readout by setting up the measurement conditions in the “multilabeling” function of Magellan software. 27 / Pack of 100; Qty. Plus . They can be used in a no-transfer protocol with suspension cells or with. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: ONE-PLATE ASSAY PROTOCOL: overnight at RT * * Depending on cell lines used, volume of lysis should be optimized. See the Cytation 5 Operator’s Manual for more information and instructions. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. FOR HTRF CERTIFIED. For more information. 96-well culture plate 384SV detection plate- Add . Lysates are then transferred to the assay plate for the detection of Total H2AX by HTRF ® reagents. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. 47 seconds for a 1536-well plate. FOR HTRF CERTIFIED READER For. Its. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed publications. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. Lysates are then transferred to the assay plate for the detection of Total Cofilin by HTRF® reagents. In particular, these parameters should be entered as defined in the table below Setup Top filter cube EX 330 / LUM (block #18) EM 620 / 665 / LUM (block #41) Light source LaserDetect flash, glow, Dual Luciferase ® Reporter and BRET assays. At Cisbio we provide microplates that help you discover better. The kit includes reagents, buffers and a microplate. Make sure the setup for Tb Cryptate is used. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. The donor and acceptor can be used to label a variety of biomolecules, for. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. EX: HTRF Emission range. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Make sure the setup for Eu 3+ Cryptate is used. The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. 96-well culture plateThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Incubate 4h at RT. For more. Read & analyse. Envision Nexus. FOR HTRF CERTIFIED READER For. Experimental details• HTRF®-Certified Reader **. $352. If not, our scientific team will help you choose the instrument that best suits your needs and budget. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersONE-PLATE ASSAY PROTOCOL: Small volume detection plate. * For HTRF microplate recommendations, please visit . No washing steps are required. (2) Forskolin activates the adenylyl cyclase enzyme and increases the intracellular level of cAMP. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. 5 EXAMPLE OF MAP PLATE . The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation. Lysates are then transferred to the assay plate for the detection of total-Rb by HTRF. FOR HTRF CERTIFIED READER . Plate 25 μL of cells in. TO PREPARE TISSUE SAMPLES: Please refer to the Technical Note “Optimize Your HTRF® Cell Signaling Assays on Tissues” onHow to use Synergy microplate readers for HTRF detection, a versatile technology for drug discovery and life science research. FOR HTRF CERTIFIED READERThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. On BMG LABTECH´s NEPHELOstar Plus, a laser diode (at 635 nm) with a highly collimated beam is used as high-intensity light source. An example of a LanthaScreen kinase assay run on a BMG LABTECH plate reader can be seen in the application note " LanthaScreen TR-FRET tyrosine kinase and protein kinase C assay ". Lysates are then transferred to ® the assay plate for the detection of Total EGFR L858R by HTRF reagents. VICTOR® Nivo™ multimode plate reader 2 | For research use only. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Detection plates from providers not listed on this. Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates. Figure 3. For more information about microplate recommendations, please visit our website STORAGE AND STABILITYONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The plate is set up for reading and images are acquired according to specified parameters. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. 2400 for 665 and 620 with white plate HTRF® Europium cryptate donor / Red acceptor readout. These are the different kinetic modes on BMG LABTECH microplate readers. • ®HTRF -Certified Reader(5) (1) IBMX is the most commonly used phosphodiesterase Pan-inhibitor. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere.